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Recombinant zonulin was proven to increase the intestinal permeability ex vivo 40 and in vitro 16

Deficiency in key autophagy molecules, such as Atg7 and Beclin-1, may contribute to DOR (Cao et al., 2017

USA 111 , 1521415219 (2014)

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Animal disease models are also flawed because of the inherent differences in the physiological nature of the BBB between animals and humans, so there is no way to tell if the results of experiments performed in animals are applicable to humans[193]